• strict warning: Non-static method view::load() should not be called statically in /var/www/pia/drupal/sites/all/modules/views/views.module on line 906.
  • strict warning: Declaration of views_handler_argument::init() should be compatible with views_handler::init(&$view, $options) in /var/www/pia/drupal/sites/all/modules/views/handlers/views_handler_argument.inc on line 744.
  • strict warning: Declaration of views_handler_filter::options_validate() should be compatible with views_handler::options_validate($form, &$form_state) in /var/www/pia/drupal/sites/all/modules/views/handlers/views_handler_filter.inc on line 607.
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  • strict warning: Declaration of views_handler_filter_boolean_operator::value_validate() should be compatible with views_handler_filter::value_validate($form, &$form_state) in /var/www/pia/drupal/sites/all/modules/views/handlers/views_handler_filter_boolean_operator.inc on line 159.
  • strict warning: Declaration of views_plugin_style_default::options() should be compatible with views_object::options() in /var/www/pia/drupal/sites/all/modules/views/plugins/views_plugin_style_default.inc on line 24.
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  • strict warning: Declaration of views_plugin_row::options_submit() should be compatible with views_plugin::options_submit(&$form, &$form_state) in /var/www/pia/drupal/sites/all/modules/views/plugins/views_plugin_row.inc on line 134.
figure

Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) is the most abundant single protein on earth and is pivotal for CO2 assimilation by all plants. In higher plants, the holoenzyme consists of eight large subunits, each with a molecular mass of 50-55 kD (identified in (b)), and eight small subunits of molecular mass 12-18 kD (not shown). Large subunits are encoded by a single gene in the chloroplast genome while a family of nuclear genes encode the small subunits.Any loss of catalytic effectivess or reduction in amount translates to slower photosynthesis and reduced growth. Tobacco plants (a) transformed with an antisense construct against Rubisco (anti-Rubisco) grow more slowly than wild types due to a 60% reduction in photosynthetic rate. Immunodetection of the large subunit polypeptide of Rubisco with an anti-Rubisco antiserum (b) shows that the anti—Rubisco transgenic plants contain less than 50% of the Rubisco detected in wild-type tobacco plants. Scale bar in (a) = 10 cm (Photograph courtesy Susanne von Caemmerer; original immunoblot courtesy Martha Ludwig)